Plasmid
pJEJAM15

Part:BBa_K678063:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-13)

pJEJAM15


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3747
    Illegal XbaI site found at 5303
    Illegal XbaI site found at 8047
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
    Illegal NotI site found at 5260
    Illegal NotI site found at 8086
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 3028
    Illegal BamHI site found at 4326
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
    Illegal XhoI site found at 4186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3747
    Illegal XbaI site found at 5303
    Illegal XbaI site found at 8047
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3747
    Illegal XbaI site found at 5303
    Illegal XbaI site found at 8047
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4588
    Illegal NgoMIV site found at 3664
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4379
    Illegal BsaI.rc site found at 1116
    Illegal BsaI.rc site found at 3378
    Illegal SapI site found at 322


Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.


DTU-Denmark-2 2011



Source

See the individual biobricks

References